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DMT-2′-O-Methyl-rG(ib) Phosphoramidite
Catalog NO.: RNA-OMEPM-003 | CAS NO.: 150780-67-9 |
Brand: BIOCAXIS
Category
Carbohydrates, Nucleosides & Nucleotides, RNA
Phosphoramidites
Synonyms:
DMT-2′-O-Me-rG(ib) amidite,
N-(2-methyl-1-oxopropyl)-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-guanosine,
3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]
Molecular Formula
C45H56N7O9P
Molecular Weight
869.94
General description
Proligo′s 2′O-Methyl RNA monomers are
compatible with fast deprotection schemes that are based on the application of
aliphatic amines, such as methylamine. The adenosine and guanosine monomers are
protected with the standard benzoyl (bz) and isobutyryl (ib) groups.2′O-Methyl
RNA is a nucleic acid analogue that is characterized by the exceptional
hybridization properties that it imparts with complimentary DNA or RNA, as well
as increased stability against enzymatic degradation compared to natural
nucleic acids. The unique combination of properties of 2′O-Methyl RNA had
found widespread use in the fields of:
Diagnostic probes
Aptamer and ribozyme development
Mixed 2′O-Methyl-RNA/DNA antisense molecule
Key Features:
High yield of crude oligonucleotides
Compatible with DNA synthesis
Can be employed together with DNA or RNA phosphoramidites
in the same synthesis to produce mixmer oligonucleotides
Recommended deprotection conditions are 8 hours at 55 °C
using concentrated ammonia solution, or with AMA (concentrated ammonia/40%
aqueous methylamine I/I, v/v) for 10 minutes at 65 °C
Purification and other downstream processing of fully
modified 2′OMethyl RNA oligonucleotides are simpler than in the case of
RNA, as no special precautions are required to provide protection against
nucleolyticdegradationDMT-2′O-Methyl-rG(ib) Phosphoramidite
Usage
Solid phase synthesis of DNA and RNA oligonucleotides in
chemical or enzymatic processes.